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PCM
Polish Collection of Microorganisms - Catalog of strains
PCM database search
Strains can be searched by strain name or number

Database:
Name
Number
begins with:
includes:
   

List of media
List of abbreviations
Selected strains with special features and applications
Definitions of pathogenic groups
Quality control strains

 

List of media
Med. 1 -- Nutrient Agar
componentsBeef Extract 10 g
Peptone 10 g
NaCl 5 g
Agar 20 g
Distilled water pH 7.2 1000 ml
Sterilization at 121oC - 20 min.
Med. 2 -- Blood Agar
componentsNutrient Agar (melted and cooled to 50oC) 900 ml
Defibrinated blood (added aseptically) 100 ml
Med. 3 -- Yeast-Glucose Agar
componentsBacto-Peptone 3 g
Beef Extract 3 g
Yeast Extract 1 g
NaCl 3 g
Glucose 20 g
Agar 20 g
Distilled water pH 6.8-6.9 1000 ml
Sterilization at 121oC - 20 min.
Med. 4 -- Yeast-Malt Agar
componentsYeast Extract 4 g
Malt Extract 10 g
Glucose 4 g
Agar 20 g
Distilled water pH 7.3 1000 ml
Med. 5 -- MYPG Medium
componentsMalt Extract 3 g
Yeast Extract 3 g
Peptone 5 g
Glucose 20 g
Agar 20 g
Distilled water 1000 ml
Med. 6 -- Mannitol Medium
componentsYeast Extract 5 g
Peptone 3 g
Mannitol 25 g
Agar 15 g
Distilled water 1000 ml
Med. 7 -- Bordet-Gengou Agar - modified
componentsBordet-Gengou Agar Base 36 g
Proteose Peptone 10 g
1% Solution of Glycerol (in distilled water) 1000 ml
After sterilization at 121oC and cooling to 45-50oC add aseptically defibrinated blood (horse, rabbit) to final concentration 15%
Med. 8 -- Tryptose Agar
componentsBacto-Tryptose (Difco) 10 g
Beef Extract 3 g
NaCl 5 g
Distilled water 1000 ml
Sterilization at 121oC - 15 min. (15% of defibrinated blood can be added)
Med. 9 -- Brucella Medium Base (Oxoid CM 169)
Med. 10 -- Thioglycollate Agar (Oxoid)
Med. 11 -- Thioglycollate Medium (Difco) - modified
componentsThioglycollate Medium (carbohydrate free) 24 g
Glucose 5.5 g
Agar 17 g
Distilled water 1000 ml
Med. 12 -- Thioglycollate-Glucose Agar with 15% of defibrinated blood
Med. 13 -- Thioglycollate-Glucose Liquid Medium
Med. 14 -- Brain Heart Infusion Agar (Difco or Oxoid)
Med. 15 -- Casitone Medium
componentsCasitone 3 g
Yeast Extract 5 g
CaCl2-2H2O 1 g
Agar 20 g
Distilled water pH 7.2 1000 ml
Med. 16 -- Serum Agar
componentsNutrient Agar with 10% of serum (horse, sheep or rabbit)
Med. 17 -- Acetobacter-Gluconobacter Medium
componentsTryptone 10 g
Yeast Extract 5 g
Glucose 20 g
K2HPO4 1 g
CaCO3 1 g
Agar 20 g
Distilled water 1000 ml
Sterilization at 121oC - 20 min.
Med. 18 -- "Chocolate" Agar
componentsBlood Agar warmed at 80oC for 3 min.
Med. 19 -- Haemophilus Medium
componentsA. Solution of G-C Medium (BBL 11275) 36 g in 500 ml of distilled water
B. Solution of Bovine Hemoglobin (BBL 11841) 10 g in 500 ml of distilled water
C. Solution of Isovitalex (BBL 11876) 1 vial in 10 ml of 10% sterile glucose
A and B should be steriliz
Med. 20 -- MRS Agar (Oxoid)
Med. 21 -- AOAC Agar (Difco)
Med. 22 -- Lowenstein-Jensen Medium
componentsKH2PO4 2.4 g
MgSO4 0.24 g
Magnesium citrate 0.6 g
Asparagine 3.6 g
Glycerol 10.0 g
Potato starch 30.0 g
Distilled water 600 ml
Autoclave at 121oC for 25 minutes, cool to 56oC and add aseptically:
Malachite green solution (2% w/v) 20 ml
Beaten eg
Med. 23 -- Bushnell-Haas Medium
componentsMgSO4-7H2O 0.2 g
CaCl2 0.02 g
KH2PO4 1.0 g
K2HPO4 1.0 g
NH4NO3 1.0 g
FeCl3 0.05 g
Cholesterol 0.3 g
Distilled water pH 7.0-7.2 1000 ml
Med. 24 -- Tomato Juice Agar
componentsTryptone 10 g
Yeast Extract 10 g
Filtered Tomato Juice 200 ml
Agar 11 g
Distilled water pH 7.2 to 1000 ml
Med. 25 -- Glycerol Agar
componentsBeef Extract 3 g
Peptone 10 g
NaCl 5 g
Agar 15 g
Glycerol 40 ml
Distilled water 1000 ml
Med. 26 -- Pseudomonas saccharophila Medium
componentsKH2PO4-Na2HPO4.M/30 1000 ml
NH4Cl 1.0 g
MgSO4.7H2O 0.5 g
FeCl3.6H2O 0.05 g
CaCl2.2H2O 0.01 g
Sucrose 2.0 g
Autoclave at 121oC - 15 min.
Med. 27 -- Azotobacter Medium I
componentsGlucose 20.0 g
K2HPO4 10.0 g
MgSO4 0.5 g
CaCO3 5.0 g
Agar 20.0 g
Distilled water 1000 ml
Med. 28 -- Azotobacter Medium II
componentsMannitol 20.0 g
K2HPO4 10.0 g
MgSO4 0.5 g
CaCO3 5.0 g
Agar 20.0 g
Distilled water 1000 ml
Med. 29 -- Beijerinckia Medium
componentsGlucose 20.0 g
K2HPO4 0.8 g
KH2PO4 0.2 g
MgSO4 0.5 g
Agar 20.0 g
Distilled water 1000 ml
Med. 30 -- Rhizobium Medium
componentsK2HPO4 0.66 g
KH2PO4 0.34 g
MgSO4 0.20 g
NaCl 0.10 g
FeSO4 0.002 g
MnSO4 0.002 g
CaCl2 0.10 g
CaCO3 5.00 g
Mannitol 10.00 g
Agar 18.00 g
Yeast Extract 1 g
Distilled water 1000 ml
Med. 31 -- Potato-Glucose Medium
components200 g of peeled and diced potatoes are boiled for 20 minutes in 500 ml of distilled water. Then filtered
The filtrate is added with:
Water to 1000 ml
Glucose 10 g
Agar 15 g
Autoclave at 115oC for 20 minutes
Med. 32 -- Med. 31 - modified I
componentsMedium 31 with:
0.2% of CaCO3
0.1% of Resazurin
0.05% of Cysteine
Med. 33 -- Med. 31 - modified II
componentsMedium 31 with:
0.5% of Yeast Extract
Med. 34 -- Oat Agar I
componentsOat meal 3.0 g
KNO3 0.2 g
K2HPO4 0.5 g
MgSO4.7H2O 0.2 g
Agar 20.0 g
Distilled water pH 7 1000 ml
Med. 35 -- Oat Agar II
componentsOat flakes 10 g
Oat meal 10 g
Agar 4 g
Tap water 1000 ml
Med. 36 -- Yeast-Starch Agar
componentsYeast Extract 4.0 g
Soluble Starch 15.0 g
K2HPO4 1.0 g
MgSO4.7H2O 0.5 g
Agar 15.0 g
Distilled water 1000 ml
Med. 37 -- Czapek-Dox Medium
componentsNaNO3 2.0 g
K2HPO4 1.0 g
KCl 0.5 g
MgSO4.7H2O 0.5 g
FeSO4 0.01 g
Sucrose 30.0 g
Agar 20.0 g
ZnSO4 0.01 g
CuSO4 0.005 g
Distilled water 1000 ml
Autoclave at 121oC - 15 min.
Med. 38 -- Bennet's Sucrose Medium
componentsMeat Extract 1 g
Yeast Extract 1 g
Casein Peptone 2 g
Sucrose 10 g
Agar 20 g
Distilled water pH 7.5-final 7.0 1000 ml
Med. 39 -- Sporulation Agar
componentsYeast Extract 1 g
Beef Extract 1 g
Tryptose 2 g
FeSO4 Trace
Glucose 10 g
Agar 20 g
Distilled water 1000 ml
Med. 40 -- Beef Liver Medium
componentsBeef liver (small pieces) 500 g
Tap water 1000 ml
Soak the liver overnight in refrigerator Remove fat of top. Autoclave at 121oC - 15 min. Filter through cheese cloth. Save the meat pieces
Add:
Peptone 10 g
K2HPO4 1 g
Adjust pH to 8. Make up volume to
Med. 41 -- Corn (Maize) Meal Agar
componentsBlend 50 g of corn meal in 800 ml of distilled water, leave overnight in refrigerator. Heat at 60oC for 1 hour. Filter and make up volume to 1 l. Add 10 g of agar and heat to dissolve, then autoclave
Med. 42 -- Malt-Sucrose Medium (M40 Y)
componentsMalt Extract 20 g
Sucrose 400 g
Yeast Extract 5 g
Agar 20 g
Distilled water 1000 ml
Med. 43 -- Mineral Salts Agar for Chaetomium
componentsNaNO3 2.0 g
MgSO4 0.5 g
KCl 0.5 g
Fe2(SO4)3.H2O 0.01 g
KH2PO4 0.14 g
K2HPO4 1.2 g
Agar 15.0 g
Yeast Extract 0.02 g
Distilled water 1000 ml
Adjust pH to 7.2
On cooled agar medium place a strip of sterile filter paper and inoculate on it
Med. 44 -- Malt-Soya Agar
componentsMalt Extract 10 g
Soya Peptone 3 g
Agar 15 g
Distilled water 1000 ml
Med. 45 -- Potato-Carrot Agar
componentsPotatoes 20 g
Carrots 20 g
Agar 15 g
Distilled water 1000 ml
Dice potatoes and carrots and cook in water for 0.5 hour
Filter through cheese cloth and add agar to filtrate
Med. 46 -- Dixon Agar
componentsMalt Extract Agar 60 g
Ox-bile 20 g
Tween 40 10 g
Glycerol mono-oleate 2.5 g
Distilled water 1000 ml