Archivum Immunologiae et Therapiae Experimentalis, 1996, 44, 45

Haptoglobin Concentration in Serum and Other Body Fluids Measured by Comparison of Its Reactivity with Hemoglobin and Concanavalin A


Abstract. Haptoglobin (Hp) concentration in normal human sera was determined either by means of the reaction with hemoglobin (Hb) or by reaction with the lectin concanavalin A (Con A). The ratio of H -Con A 1.02±0.68 g/1) to Hp-Hb (1.10±0.58 g/1) equal 0.96±0.32 was found to be characteristic for sera from healthy subjects. Measurements were also carried out for some pathological body fluids. Values of the ratio Hp-Con A/Hp-Hb in ascitic fluid, pleural effusion and cerebrospinal fluid were 1.72± 1.13 0.42±023 and 0.15±0.18, respectively. Haptoglobin present in urine and saliva was not recognized by Con A, whereas could form a complex with hemoglobin. Hp concentration determined by this method reached 2 mg/l.

Keywords: haptoglobin; concanavalin; glycosylation.lation.