Antigenicity of Human and Horse Myoglobins
IWONA E. KOCHANOWSKA, MARIANNA KUROPATWA, ANDRZEJ RAPAK and APOLINARY SZEWCZUK
Abstract. Rabbit antisera against human myoglobin and horse myoglobin cross-reacted with both myoglobins but only one of them recognized human hemoglobin. Two mouse monoclonal antibodies anti-human myo- globin were obtained, but only one of them (No. 49) cross-reacted with horse myoglobin. Antibody No. 49 and rabbit antibodies reacted also with apo-, FITC- and treated with hydrochloric acid or TPCK-trypsin horse myoglobin, but their binding to myoglobin pretreated with NaOH was reduced. Thirteen peptides overlapping sequence of human myoglobin were synthesized on polyethylene pins. Rabbit and mouse polyclonal an- tibodies reacted with some of these peptides but no reaction was noted with mouse monoclonal antibodies. Two monoclonal antibodies were applied for specific immunoassay of human myoglobin.
Keywords: antigenicity, human myoglobin, horse myoglobin, immunoassay of myoglobin, polyclonal and monoclonal antibodies.bodies.