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Archivum Immunologiae et Therapiae Experimentalis, 1996, 44, 33


Antigenicity of Human and Horse Myoglobins

IWONA E. KOCHANOWSKA, MARIANNA KUROPATWA, ANDRZEJ RAPAK and APOLINARY SZEWCZUK

Abstract. Rabbit antisera against human myoglobin and horse myoglobin cross-reacted with both myoglobins but only one of them recognized human hemoglobin. Two mouse monoclonal antibodies anti-human myo- globin were obtained, but only one of them (No. 49) cross-reacted with horse myoglobin. Antibody No. 49 and rabbit antibodies reacted also with apo-, FITC- and treated with hydrochloric acid or TPCK-trypsin horse myoglobin, but their binding to myoglobin pretreated with NaOH was reduced. Thirteen peptides overlapping sequence of human myoglobin were synthesized on polyethylene pins. Rabbit and mouse polyclonal an- tibodies reacted with some of these peptides but no reaction was noted with mouse monoclonal antibodies. Two monoclonal antibodies were applied for specific immunoassay of human myoglobin.

Keywords: antigenicity, human myoglobin, horse myoglobin, immunoassay of myoglobin, polyclonal and monoclonal antibodies.bodies.