General title of the scientific activity:
“Immunochemical and genetic studies on human glycophorin and other proteins, active in immunological system”.
Individual subjects are as follows:
Invasion of human erythrocytes by Plasmodium falciparum malaria parasites
The studies regard structure and interaction of BAEBL ligand of malaria parasite with glycophorin C, a receptor from erythrocyte membrane. Knowing the place of interaction of ligand and receptor will help to understand the invasion process and may have practical consequences for inhibition of this process.
Involvement of carcinoembryonic antigen (CEA) in intercellular interactions
It was shown that CEA acts as homo/heterotypic intercellular adhesion molecule. The studies regard the mechanism of these interactions. They deal with determination of active peptidic fragments within Ig-like domains of CEA molecule and with determination of individual amino acids, which take part in homotypic adhesion.
Molecular characteristics of Duffy antigen
Duffy antigen is a glycosylated protein of human erythrocyte membrane. The studies regard characterization of epitopes (protein-type and protein/sugar type), recognized by monoclonal antibodies in Duffy molecule and in other blood-group antigens. The basic method is pepscan, which involves short peptides, synthesized on plastic pins, having defined amino acid sequence.
Production and characteristics of recombinant antibodies, recognizing blood group antigens
Methodology was elaborated during production of antibodies, recognizing glycophorin A from human erythrocyte membrane. The subject of investigations is the role of amino acid sequence of variable regions in recognizing defined antigen.
Molecular characteristics of rare blood group antigens
The studies are performed on very rare NOR erythrocytes, in which not typical sugar sequence was identified. The studies are based on determination of glycosyltransferase-gen mutations.
Sugar moiety of serum IgG in pathological cases
The studies regard IgG, isolated from serum of rheumatoid (RA) and psoriatic (PA) arthritis patients. The basic difference in IgG sugar moiety is status of galactosylation of conservative N-glycans in IgG heavy chains. Analyses are performed using ELISA test, sugar GC-MS analysis and biosensor BIAcore.