The general subject of our research concerns the role of proteins which participate in cGMP- and cAMP-dependent intracellular signaling. We are interested in relations between the activities of enzymes (cyclases, phosphodiesterases, protein kinases) constituting the particular pathway and activities of proinflammatory transcription factors.
Our studies also concern signaling by the receptor for neurotrophin-3 (TrkC) that regulates the development of neuronal cells. The model cells (neural and neuroendocrine origin cells) are genetically engineered to express inducible TrkC and activation of signaling proteins and genes following TrkC activation are examined.
In our studies we employ assays which measure activities of guanylyl cyclases, phosphodiesterases, and kinases; we also use RT-PCR, EMSA, reporter assays, and basic bio- and-immunochemical methods.